Electroporation-mediated infection of tobacco leaf protoplasts with tobacco...
AbstractConditions were established for the introduction of both tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) RNAs into tobacco mesophyll protoplasts by electroporation. The proportion of...
View ArticleAn improved method for electroporation in plant protoplasts: infection of...
AbstractConditions of electroporation were optimized for introduction of tobacco mosaic virus (TMV) particles into tobacco mesophyll protoplasts (Nicotiana tabacum L. cv. Petit Havana SR1). Compared...
View ArticleNucleotide sequence analysis of two nuclear inclusion body and coat protein...
SummaryRecombinant DNA molecules containing cDNA of a sweet potato feathery mottle virus severe strain (SPFMV-S) RNA genome were constructed and the partial nucleotide sequences were determined for...
View ArticleComplete nucleotide sequence and genome organization of sweet potato feathery...
SummaryThe complete nucleotide sequence of a sweet potato feathery mottle virus severe strain (SPFMV-S) genomic RNA was determined from overlapping cDNA clones and by directly sequencing viral RNA. The...
View ArticleCellular dissection of the degradation pattern of cortical cell death during...
Abstract. Cellular events which occur prior to cell collapse were examined in the root cortex of rice (Oryza sativa L.) during aerenchyma formation. Cell collapse started at a specific position in the...
View ArticleNucleotide sequence analysis of the 3′ terminal region of a wasabi strain of...
SummaryThe 3′ terminal 2378 nucleotides of a wasabi strain of crucifer tobamovirus (CTMV-W) infectious to crucifer plants was determined. This includes the 3′ non-coding region of 235 nucleotides, coat...
View ArticleThe genome structure of kyuri green mottle mosaic tobamovirus and its...
Summary. The genome of the Y strain of kyuri green mottle mosaic virus (KGMMV-Y) has been completely sequenced. Its genomic structure and sequence show it to be a typical tobamovirus, that is closest...
View ArticleVirus resistance in transgenic sweetpotato [Ipomoea batatas L. (Lam)]...
Abstract One of the most-serious diseases of sweet potato [Ipomoea batatas (L.) Lam] is russet crack disease caused by sweet potato feathery mottle virus (SPFMV). We constructed an expression vector...
View ArticleA receptor-like protein kinase with a lectin-like domain from lombardy...
Abstract. Plant receptor-like protein kinases (RLKs) are thought to be involved in various cellular processes mediated via signal transduction pathways. To clarify the initial step in such a signal...
View ArticleMicroarray Analysis of Gene Expression in Rice Treated with Probenazole, a...
AbstractProbenazole (3-allyloxy-1,2-benzisothiazole-1,1-dioxide, PBZ) is a chemical inducer of resistance against plant pathogens and has long been practically used for protecting rice plants against a...
View ArticleKonjak mosaic virus: the complete nucleotide sequence of the genomic RNA and...
Summary.Konjak mosaic virus (KoMV) belongs to the genus Potyvirus, family Potyviridae. The complete nucleotide sequence of KoMV F isolate (KoMV F) was determined. The genome is 9,544 nucleotides long...
View ArticleEthical and welfare considerations when using cephalopods as experimental...
AbstractWhen using cephalopods as experimental animals, a number of factors, including morality, quality of information derived from experiments, and public perception, drives the motivation to...
View ArticleBilateral brachial pull-through technique for stenting in a patient with...
AbstractStenting for stenosis of the proximal vertebral artery (VA) is commonly performed via a femoral approach. However, iliofemoral occlusive disease such as arteriosclerosis obliterans sometimes...
View ArticleEffect of deferoxamine-activated hypoxia inducible factor-1 on the brainstem...
AbstractBackgroundHypoxia inducible factor-1 (HIF-1) is a transcription factor that regulates the expression of various neuroprotective genes. The goal of this study was to clarify the relationship...
View ArticleRice necrosis mosaic virus , a fungal transmitted Bymovirus : complete...
AbstractThe complete nucleotide sequence of Rice necrosis mosaic virus (RNMV) RNA1 was determined to be 7178 nt long with one large open reading frame, potentially encoding a polyprotein of 258 kDa...
View ArticleAnalysis of rice RNA - dependent RNA polymerase 6 ( OsRDR6 ) gene in response...
AbstractRNA-dependent RNA polymerases (RDRs) play key roles in gene silencing. The rice RDR6 gene was analyzed in response to viral, bacterial and fungal pathogens, after inoculation of a rice mutant...
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